KMID : 1007520190280051535
|
|
Food Science and Biotechnology 2019 Volume.28 No. 5 p.1535 ~ p.1542
|
|
Comparative anti-thrombotic activity and haemorrhagic adverse effect of nattokinase and tissue-type plasminogen activator
|
|
Guo Haiyu
Ban Young-Hwan Cha Ye-Seul An Eun-Suk Choi Ji-Eun Seo Da-Woom Park Dong-Sun Choi Ehn-Kyoung Kim Yun-Bae
|
|
Abstract
|
|
|
Anti-thrombotic activity and safety of nattokinase, an enzyme produced by Bacillus subtilis during soybean fermentation, were investigated in comparison with tissue-type plasminogen activator (t-PA). Carotid arterial thrombosis was produced with a FeCl3-soaked paper, followed by intravenous injection of nattokinase or t-PA. Nattokinase and t-PA delayed thrombus formation, near-fully (>?90%) inhibiting at 75 and 8.5 mg/kg, respectively. As adverse effects, t-PA induced petechial haemorrhage at 10 mg/kg in the lungs and thymus, and extensive bleeding at 20 mg/kg. Nattokinase also caused pulmonary haemorrhage from 300 mg/kg. Collectively, the standard safety margins (SSMs) for t-PA and nattokinase were calculated to be 1.2 and 4.0, respectively. Combinational treatment with dexamethasone (2 mg/kg) increased the efficacy and safety of t-PA and nattokinase, widening their SSMs to 2.4 and 8.0, respectively. The results indicate that nattokinase delayed thrombus formation and dissolved thrombi, and that nattokinase could be a good candidate anti-thrombotic agent with relatively-low haemorrhagic risk.
|
|
KEYWORD
|
|
Nattokinase, Tissue-type plasminogen activator, Dexamethasone, Thrombosis, Haemorrhage
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|